Identification of a novel SNP mutation causing drop-out alleles in a paternity test using combined nest and touch-down PCR with Sanger sequencing

• Main Authors: Hongbo Wang (Author), Botong Shi (Author), Liang Guo (Author), Yingnan Zou (Author), Bo Liu (Author), Jun Yao (Author), Baojie Wang (Author), Zhengdong Wang (Author), Xin Li (Author), Gang Mu (Author), Fu Ren (Author)
• Format: Book
• Published: Taylor & Francis Group, 2024-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Background Short tandem repeat (STR) markers are widely used in forensic DNA analysis due to their ability to provide automated and standardised typing. However, incorrect STR typing can have a significant impact on forensic outcomes.Aim In this study, we detected drop-out alleles at the SE33 locus...