Synthetic Design of Asymmetric miRNA with an Engineered 3' Overhang to Improve Strand Selection

• Main Authors: Sandeep Kadekar (Author), Ganesh N. Nawale (Author), Kira Karlsson (Author), Cecilia Ålander (Author), Oommen P. Oommen (Author), Oommen P. Varghese (Author)
• Format: Book
• Published: Elsevier, 2019-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

We developed a novel miRNA design that significantly improves strand selection within the RISC complex by engineering the 3' end by adding extra nucleotides. Addition of seven nucleotides at the 3' ends of the miR or miR* strand resulted in a thermodynamic asymmetry at either of the two en...