Morphological Change by Overexpression of D385A Dominant Negative Presenilin 1 in Human Neuroblastoma SH-SY5Y Cells

• Main Authors: Daiju Tsuchiya (Author), Yoshihisa Kitamura (Author), Kazuyuki Takata (Author), Takashi Taniguchi (Author), Kengo Uemura (Author), Hiroaki Miki (Author), Tadaomi Takenawa (Author), Shun Shimohama (Author)
• Format: Book
• Published: Elsevier, 2006-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Presenilin 1 (PS1) is a multifunctional protein, and its mutations are highly related to familial Alzheimer's disease (AD). In this study, we examined the effects of PS1 overexpression on neuronal morphology using SH-SY5Y cells. Overexpression of dominant-negative D385A PS1 induced morphological change and impairment of neurite formation, while those of wild-type and pathogenic P117L mutant PS1 did not change cellular morphology compared with native cells. Moreover, filopodium-formation-related proteins were decreased only in cells overexpressing D385A PS1. Therefore, PS1 may be involved in neuritogenesis and morphological change in SH-SY5Y cells, and P117L mutation may linked to AD by different mechanisms. Keywords:: presenilin 1, SH-SY5Y cell, neuritogenesis

Item Description

1347-8613
10.1254/jphs.SC0060118