Morphological Change by Overexpression of D385A Dominant Negative Presenilin 1 in Human Neuroblastoma SH-SY5Y Cells
Presenilin 1 (PS1) is a multifunctional protein, and its mutations are highly related to familial Alzheimer's disease (AD). In this study, we examined the effects of PS1 overexpression on neuronal morphology using SH-SY5Y cells. Overexpression of dominant-negative D385A PS1 induced morphologica...
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| Main Authors: | , , , , , , , |
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| Format: | Book |
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Elsevier,
2006-01-01T00:00:00Z.
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| 001 | doaj_d864c7f69f124a9e9bafc27d90d99f6f | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Daiju Tsuchiya |e author |
| 700 | 1 | 0 | |a Yoshihisa Kitamura |e author |
| 700 | 1 | 0 | |a Kazuyuki Takata |e author |
| 700 | 1 | 0 | |a Takashi Taniguchi |e author |
| 700 | 1 | 0 | |a Kengo Uemura |e author |
| 700 | 1 | 0 | |a Hiroaki Miki |e author |
| 700 | 1 | 0 | |a Tadaomi Takenawa |e author |
| 700 | 1 | 0 | |a Shun Shimohama |e author |
| 245 | 0 | 0 | |a Morphological Change by Overexpression of D385A Dominant Negative Presenilin 1 in Human Neuroblastoma SH-SY5Y Cells |
| 260 | |b Elsevier, |c 2006-01-01T00:00:00Z. | ||
| 500 | |a 1347-8613 | ||
| 500 | |a 10.1254/jphs.SC0060118 | ||
| 520 | |a Presenilin 1 (PS1) is a multifunctional protein, and its mutations are highly related to familial Alzheimer's disease (AD). In this study, we examined the effects of PS1 overexpression on neuronal morphology using SH-SY5Y cells. Overexpression of dominant-negative D385A PS1 induced morphological change and impairment of neurite formation, while those of wild-type and pathogenic P117L mutant PS1 did not change cellular morphology compared with native cells. Moreover, filopodium-formation-related proteins were decreased only in cells overexpressing D385A PS1. Therefore, PS1 may be involved in neuritogenesis and morphological change in SH-SY5Y cells, and P117L mutation may linked to AD by different mechanisms. Keywords:: presenilin 1, SH-SY5Y cell, neuritogenesis | ||
| 546 | |a EN | ||
| 690 | |a Therapeutics. Pharmacology | ||
| 690 | |a RM1-950 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Journal of Pharmacological Sciences, Vol 102, Iss 3, Pp 354-358 (2006) | |
| 787 | 0 | |n http://www.sciencedirect.com/science/article/pii/S1347861319343804 | |
| 787 | 0 | |n https://doaj.org/toc/1347-8613 | |
| 856 | 4 | 1 | |u https://doaj.org/article/d864c7f69f124a9e9bafc27d90d99f6f |z Connect to this object online. |