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Comparison of diagnostic performance of RT-qPCR, RT-LAMP and IgM/IgG rapid tests for detection of SARS-CoV-2 among healthcare workers in Brazil

Background: COVID-19 has become a major public health problem after the outbreak caused by SARS-CoV-2 virus. Great efforts to contain COVID-19 transmission have been applied worldwide. In this context, accurate and fast diagnosis is essential. Methods: In this prospective study, we evaluated the cli...

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Main Authors: Wilma Patrícia de Oliveira Santos Bernardes (Author), Thais Garcia Santos (Author), Nubia Monteiro Gonçalves Soares Fernandes (Author), Thais Bárbara de Souza Silva (Author), Mateus Westin (Author), Taynãna César Simões (Author), Eduardo Fernandes e Silva (Author), Breno Magalhães Alves (Author), Israel Molina (Author), Mariana de Carvalho Melo (Author), Rubens Lima do Monte-Neto (Author), Rosiane A. da Silva-Pereira (Author), Pedro Augusto Alves (Author), Cristina Toscano Fonseca (Author)
Format: Book
Published: Elsevier, 2023-07-01T00:00:00Z.
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Summary:Background: COVID-19 has become a major public health problem after the outbreak caused by SARS-CoV-2 virus. Great efforts to contain COVID-19 transmission have been applied worldwide. In this context, accurate and fast diagnosis is essential. Methods: In this prospective study, we evaluated the clinical performance of three different RNA-based molecular tests - RT-qPCR (Charité protocol), RT-qPCR (CDC (USA) protocol) and RT-LAMP - and one rapid test for detecting anti-SARS-CoV-2 IgM and IgG antibodies. Results: Our results demonstrate that RT-qPCR using the CDC (USA) protocol is the most accurate diagnostic test among those evaluated, while oro-nasopharyngeal swabs are the most appropriate biological sample. RT-LAMP was the RNA-based molecular test with lowest sensitivity while the serological test presented the lowest sensitivity among all evaluated tests, indicating that the latter test is not a good predictor of disease in the first days after symptoms onset. Additionally, we observed higher viral load in individuals who reported more than 3 symptoms at the baseline. Nevertheless, viral load had not impacted the probability of testing positive for SARS-CoV-2. Conclusion: Our data indicates that RT-qPCR using the CDC (USA) protocol in oro-nasopharyngeal swabs samples should be the method of choice to diagnosis COVID-19.
Item Description:1876-0341
10.1016/j.jiph.2023.05.009

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