Implementation of a Design of Experiments to Improve Periplasmic Yield of Functional ScFv Antibodies in a Phage Display Platform

• Главные авторы: Marjan Abri Aghdam (Автор), Mohammad Reza Tohidkia (Автор), Elham Ghamghami (Автор), Asadollah Ahmadikhah (Автор), Morteza Khanmahamadi (Автор), Behzad Baradaran (Автор), Ahad Mokhtarzadeh (Автор)
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• Опубликовано: Tabriz University of Medical Sciences, 2022-05-01T00:00:00Z.
• Предметы: article
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Purpose: Production of functional recombinant antibody fragments in the periplasm of E. coli isa prerequisite step to achieve sufficient reagent for preclinical studies. Thus, the cost-effectiveand lab-scale production of antibody fragments demands the optimization of culture conditions.Methods: The...