Implementation of a Design of Experiments to Improve Periplasmic Yield of Functional ScFv Antibodies in a Phage Display Platform

• 主要な著者: Marjan Abri Aghdam (著者), Mohammad Reza Tohidkia (著者), Elham Ghamghami (著者), Asadollah Ahmadikhah (著者), Morteza Khanmahamadi (著者), Behzad Baradaran (著者), Ahad Mokhtarzadeh (著者)
• フォーマット: 図書
• 出版事項: Tabriz University of Medical Sciences, 2022-05-01T00:00:00Z.
• 主題: article
• オンライン･アクセス: Connect to this object online.

Purpose: Production of functional recombinant antibody fragments in the periplasm of E. coli isa prerequisite step to achieve sufficient reagent for preclinical studies. Thus, the cost-effectiveand lab-scale production of antibody fragments demands the optimization of culture conditions.Methods: The...