Implementation of a Design of Experiments to Improve Periplasmic Yield of Functional ScFv Antibodies in a Phage Display Platform

• Main Authors: Marjan Abri Aghdam (Author), Mohammad Reza Tohidkia (Author), Elham Ghamghami (Author), Asadollah Ahmadikhah (Author), Morteza Khanmahamadi (Author), Behzad Baradaran (Author), Ahad Mokhtarzadeh (Author)
• Format: Book
• Published: Tabriz University of Medical Sciences, 2022-05-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Purpose: Production of functional recombinant antibody fragments in the periplasm of E. coli isa prerequisite step to achieve sufficient reagent for preclinical studies. Thus, the cost-effectiveand lab-scale production of antibody fragments demands the optimization of culture conditions.Methods: The...