Efficient Non-Viral Integration and Stable Gene Expression in Multipotent Adult Progenitor Cells

• প্রধান লেখক: Andrew Wilber (লেখক), Fernando Ulloa Montoya (লেখক), Luke Hammer (লেখক), Branden S. Moriarity (লেখক), Aron M. Geurts (লেখক), David A. Largaespada (লেখক), Catherine M. Verfaillie (লেখক), R. Scott McIvor (লেখক), Uma Lakshmipathy (লেখক)
• বিন্যাস: গ্রন্থ
• প্রকাশিত: Hindawi Limited, 2011-01-01T00:00:00Z.
• বিষয়গুলি: article
• অনলাইন ব্যবহার করুন: Connect to this object online.

Non-viral integrating systems, PhiC31 phage integrase (ϕC31), and Sleeping Beauty transposase (SB), provide an effective method for ex vivo gene delivery into cells. Here, we used a plasmid-encoding GFP and neomycin phosphotransferase along with recognition sequences for both ϕC31 and SB integrating...