Efficient Non-Viral Integration and Stable Gene Expression in Multipotent Adult Progenitor Cells

• Main Authors: Andrew Wilber (Author), Fernando Ulloa Montoya (Author), Luke Hammer (Author), Branden S. Moriarity (Author), Aron M. Geurts (Author), David A. Largaespada (Author), Catherine M. Verfaillie (Author), R. Scott McIvor (Author), Uma Lakshmipathy (Author)
• Format: Book
• Published: Hindawi Limited, 2011-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Non-viral integrating systems, PhiC31 phage integrase (ϕC31), and Sleeping Beauty transposase (SB), provide an effective method for ex vivo gene delivery into cells. Here, we used a plasmid-encoding GFP and neomycin phosphotransferase along with recognition sequences for both ϕC31 and SB integrating...