Low temperature preservation of bovine ovaries on in vitro development of oocytes

• Main Authors: Sung Woo Kim (Author), Min Su Kim (Author), Chan-Lan Kim (Author), Dongkyo Kim (Author), Namtae Kim (Author), Hwan-Hoo Seong (Author)
• Format: Book
• Published: The Korean Society of Animal Reproduction and Biotechnology, 2016-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

During the ovary preservation in low temperature, the cumulus oocyte complexes(COCs) lose their developmental competences after in vitro fertilization. We used phosphate-buffered saline (PBS) as a basic solutions of at various temperatures (25, 15 or 5 °C) and supplemented them with 1mM glucose and...