Reduced insulin action in muscle of high fat diet rats over the diurnal cycle is not associated with defective insulin signaling
Objective: Energy metabolism and insulin action follow a diurnal rhythm. It is therefore important that investigations into dysregulation of these pathways are relevant to the physiology of this diurnal rhythm. Methods: We examined glucose uptake, markers of insulin action, and the phosphorylation o...
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Elsevier,
2019-07-01T00:00:00Z.
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|---|---|---|---|
| 001 | doaj_db9fca57c93a4a79959a2db147f25fc7 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Lewin Small |e author |
| 700 | 1 | 0 | |a Amanda E. Brandon |e author |
| 700 | 1 | 0 | |a Benjamin L. Parker |e author |
| 700 | 1 | 0 | |a Vinita Deshpande |e author |
| 700 | 1 | 0 | |a Azrah F. Samsudeen |e author |
| 700 | 1 | 0 | |a Greg M. Kowalski |e author |
| 700 | 1 | 0 | |a Jane Reznick |e author |
| 700 | 1 | 0 | |a Donna L. Wilks |e author |
| 700 | 1 | 0 | |a Elaine Preston |e author |
| 700 | 1 | 0 | |a Clinton R. Bruce |e author |
| 700 | 1 | 0 | |a David E. James |e author |
| 700 | 1 | 0 | |a Nigel Turner |e author |
| 700 | 1 | 0 | |a Gregory J. Cooney |e author |
| 245 | 0 | 0 | |a Reduced insulin action in muscle of high fat diet rats over the diurnal cycle is not associated with defective insulin signaling |
| 260 | |b Elsevier, |c 2019-07-01T00:00:00Z. | ||
| 500 | |a 2212-8778 | ||
| 500 | |a 10.1016/j.molmet.2019.04.006 | ||
| 520 | |a Objective: Energy metabolism and insulin action follow a diurnal rhythm. It is therefore important that investigations into dysregulation of these pathways are relevant to the physiology of this diurnal rhythm. Methods: We examined glucose uptake, markers of insulin action, and the phosphorylation of insulin signaling intermediates in muscle of chow and high fat, high sucrose (HFHS) diet-fed rats over the normal diurnal cycle. Results: HFHS animals displayed hyperinsulinemia but had reduced systemic glucose disposal and lower muscle glucose uptake during the feeding period. Analysis of gene expression, enzyme activity, protein abundance and phosphorylation revealed a clear diurnal regulation of substrate oxidation pathways with no difference in Akt signaling in muscle. Transfection of a constitutively active Akt2 into the muscle of HFHS rats did not rescue diet-induced reductions in insulin-stimulated glucose uptake. Conclusions: These studies suggest that reduced glucose uptake in muscle during the diurnal cycle induced by short-term HFHS-feeding is not the result of reduced insulin signaling. Keywords: Insulin action, Glucose uptake, Skeletal muscle, Insulin signaling, Diurnal rhythms, Phosphoproteomics | ||
| 546 | |a EN | ||
| 690 | |a Internal medicine | ||
| 690 | |a RC31-1245 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Molecular Metabolism, Vol 25, Iss , Pp 107-118 (2019) | |
| 787 | 0 | |n http://www.sciencedirect.com/science/article/pii/S2212877819302728 | |
| 787 | 0 | |n https://doaj.org/toc/2212-8778 | |
| 856 | 4 | 1 | |u https://doaj.org/article/db9fca57c93a4a79959a2db147f25fc7 |z Connect to this object online. |