Engineering an adenine base editor in human embryonic stem cells with minimal DNA and RNA off-target activities

• Main Authors: Zhenwu Zhang (Author), Wanyu Tao (Author), Shisheng Huang (Author), Wenjun Sun (Author), Yue Wang (Author), Wen Jiang (Author), Xingxu Huang (Author), Chao-Po Lin (Author)
• Format: Book
• Published: Elsevier, 2022-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Genome editing in pluripotent stem cells (PSCs) using CRISPR technology holds great promise for therapeutic applications. Yet, it has been reported that Cas9-mediated cleavage could cause large deletions or rearrangements of DNA, and the selection of edited PSCs could acquire p53 mutations. Adenine...