CRISPR/Cas9 RET Gene Knockout in Medullary Thyroid Carcinoma Cell-lines: Optimization and Validation

• Main Authors: Marjan Zarif-Yeganeh (Author), Dariush D Farhud (Author), Azam Rahimpour (Author), Sara Sheikholeslami (Author), Setareh Shivaei (Author), Mehdi Hedayati (Author)
• Format: Book
• Published: Tehran University of Medical Sciences, 2022-05-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Background: Medullary Thyroid Cancer (MTC) is a very aggressive type of thyroid carcinoma. Mutation in RET proto-oncogene is demonstrated in MTC development. We aimed to knock-out of RET-oncogene using CRISPR/Cas9 genome editing method in MTC cell-lines. Methods: This research was conducted in Shahi...