Development of a single-tube one-step RT-LAMP assay to detect the Chikungunya virus genome.

• Main Authors: Benjamin Lopez-Jimena (Author), Stefanie Wehner (Author), Graham Harold (Author), Mohammed Bakheit (Author), Sieghard Frischmann (Author), Michaël Bekaert (Author), Oumar Faye (Author), Amadou Alpha Sall (Author), Manfred Weidmann (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2018-05-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

A single-tube one-step real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid detection of chikungunya virus (CHIKV) targeting the conserved 6K-E1 target region was developed. The assay was validated with sera collected from a CHIKV outbreak in Senegal in 20...