Expression of the H₂O₂ Biosensor roGFP-Tpx1.C169S in Fission and Budding Yeasts and Jurkat Cells to Compare Intracellular H₂O₂ Levels, Transmembrane Gradients, and Response to Metals

• Main Authors: Laura de Cubas (Author), Jorge Mallor (Author), Víctor Herrera-Fernández (Author), José Ayté (Author), Rubén Vicente (Author), Elena Hidalgo (Author)
• Format: Bog
• Udgivet: MDPI AG, 2023-03-01T00:00:00Z.
• Fag: article
• Online adgang: Connect to this object online.

Intracellular hydrogen peroxide (H₂O₂) levels can oscillate from low, physiological concentrations, to intermediate, signaling ones, and can participate in toxic reactions when overcoming certain thresholds. Fluorescent protein-based reporters to measure...