Methotrexate mediates the integrity of intestinal stem cells partly through nitric oxide-dependent Wnt/β-catenin signaling in methotrexate-induced rat ileal mucositis

• Main Authors: Maiko Machida (Author), Takuji Machida (Author), Masaki Kikuchi (Author), Ayaka Shimizu (Author), Syunsuke Ida (Author), Yoshiki Tawaraya (Author), Risa Kato (Author), Keisuke Haramaki (Author), Kaori Yama (Author), Saki Shiga (Author), Masahiko Hirafuji (Author), Kenji Iizuka (Author)
• Format: Book
• Published: Elsevier, 2022-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

This study aimed to elucidate the role of nitric oxide (NO) in intestinal stem cells in methotrexate-induced ileal mucositis in rats. Methotrexate induced the mRNA expressions of the Wnt/β-catenin target genes Wnt3a, Sox9, and Lgr5 and the Wnt-antagonist gene sFRP-1 and the protein expressions of Lg...