Molecular assays for determining Mycobacterium leprae viability in tissues of experimentally infected mice.

• Main Authors: Grace L Davis (Author), Nashone A Ray (Author), Ramanuj Lahiri (Author), Thomas P Gillis (Author), James L Krahenbuhl (Author), Diana L Williams (Author), Linda B Adams (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2013-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

BACKGROUND: The inability of Mycobacterium leprae to grow on axenic media has necessitated specialized techniques in order to determine viability of this organism. The purpose of this study was to develop a simple and sensitive molecular assay for determining M. leprae viability directly from infect...