Dihydrofolate reductase-like protein inactivates hemiaminal pharmacophore for self-resistance in safracin biosynthesis

• Autori principali: Ning Shao (Autore), Xueyang Ma (Autore), Ying-Ying Zhang (Autore), Donghui Yang (Autore), Ming Ma (Autore), Gong-Li Tang (Autore)
• Natura: Libro
• Pubblicazione: Elsevier, 2023-03-01T00:00:00Z.
• Soggetti: article
• Accesso online: Connect to this object online.

Dihydrofolate reductase (DHFR), a housekeeping enzyme in primary metabolism, has been extensively studied as a model of acid-base catalysis and a clinic drug target. Herein, we investigated the enzymology of a DHFR-like protein SacH in safracin (SAC) biosynthesis, which reductively inactivates hemia...