Tracking Radiolabeled Endothelial Microvesicles Predicts Their Therapeutic Efficacy: A Proof-of-Concept Study in Peripheral Ischemia Mouse Model Using SPECT/CT Imaging

• Main Authors: Romain Giraud (Author), Anaïs Moyon (Author), Stéphanie Simoncini (Author), Anne-Claire Duchez (Author), Vincent Nail (Author), Corinne Chareyre (Author), Ahlem Bouhlel (Author), Laure Balasse (Author), Samantha Fernandez (Author), Loris Vallier (Author), Guillaume Hache (Author), Florence Sabatier (Author), Françoise Dignat-George (Author), Romaric Lacroix (Author), Benjamin Guillet (Author), Philippe Garrigue (Author)
• Format: Book
• Published: MDPI AG, 2022-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Microvesicles, so-called endothelial large extracellular vesicles (LEVs), are of great interest as biological markers and cell-free biotherapies in cardiovascular and oncologic diseases. However, their therapeutic perspectives remain limited due to the lack of reliable data regarding their systemic biodistribution after intravenous administration. Methods: Applied to a mouse model of peripheral ischemia, radiolabeled endothelial LEVs were tracked and their in vivo whole-body distribution was quantified by microSPECT/CT imaging. Hindlimb perfusion was followed by LASER Doppler and motility impairment function was evaluated up to day 28 post-ischemia. Results: Early and specific homing of LEVs to ischemic hind limbs was quantified on the day of ischemia and positively correlated with reperfusion intensity at a later stage on day 28 after ischemia, associated with an improved motility function. Conclusions: This concept is a major asset for investigating the biodistribution of LEVs issued from other cell types, including cancer, thus partly contributing to better knowledge and understanding of their fate after injection.

Item Description

10.3390/pharmaceutics14010121
1999-4923