Evaluation of RNA Amplification Methods to Improve DC Immunotherapy Antigen Presentation and Immune Response

• Main Authors: Jacoba G Slagter-Jäger (Author), Alexa Raney (Author), Whitney E Lewis (Author), Mark A DeBenedette (Author), Charles A Nicolette (Author), Irina Y Tcherepanova (Author)
• Format: Book
• Published: Elsevier, 2013-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Dendritic cells (DCs) transfected with total amplified tumor cell RNA have the potential to induce broad antitumor immune responses. However, analytical methods required for quantitatively assessing the integrity, fidelity, and functionality of the amplified RNA are lacking. We have developed a seri...