Viridot: An automated virus plaque (immunofocus) counter for the measurement of serological neutralizing responses with application to dengue virus.

• Main Authors: Leah C Katzelnick (Author), Ana Coello Escoto (Author), Benjamin D McElvany (Author), Christian Chávez (Author), Henrik Salje (Author), Wensheng Luo (Author), Isabel Rodriguez-Barraquer (Author), Richard Jarman (Author), Anna P Durbin (Author), Sean A Diehl (Author), Derek J Smith (Author), Stephen S Whitehead (Author), Derek A T Cummings (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2018-10-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The gold-standard method for quantifying neutralizing antibody responses to many viruses, including dengue virus (DENV), is the plaque reduction neutralization test (PRNT, also called the immunofocus reduction neutralization test). The PRNT conducted on 96-well plates is high-throughput and requires...