Attachment, Growth, and Detachment of Human Mesenchymal Stem Cells in a Chemically Defined Medium

• Main Authors: Denise Salzig (Author), Jasmin Leber (Author), Katharina Merkewitz (Author), Michaela C. Lange (Author), Natascha Köster (Author), Peter Czermak (Author)
• Format: Book
• Published: Hindawi Limited, 2016-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The manufacture of human mesenchymal stem cells (hMSCs) for clinical applications requires an appropriate growth surface and an optimized, preferably chemically defined medium (CDM) for expansion. We investigated a new protein/peptide-free CDM that supports the adhesion, growth, and detachment of an...