Successful production of offspring derived from mouse zygotes vitrified with carboxylated ε-poly-L-lysine and polyvinyl alcohol without serum

• Main Authors: Midori ISHII (Author), Maki KAMOSHITA (Author), Yoshihiro KURIHARA (Author), Kazuaki MATSUMURA (Author), Suong-Hyu HYON (Author), Junya ITO (Author), Naomi KASHIWAZAKI (Author)
• Format: Book
• Published: The Society for Reproduction and Development, 2022-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The vitrification of zygotes is important for their use as donors for generating genome-edited mice. We previously reported the successful vitrification of mouse zygotes using carboxylated ε-poly-L-lysine (COOH-PLL). However, this vitrification solution contains fetal calf serum (FCS), which contain...