Effect of oxygen hyperbaric therapy on malondialdehyde levels in saliva of periodontitis patients with type 2 diabetes mellitus

• Main Authors: Dian Mulawarmanti (Author), Widyastuti Widyastuti (Author)
• Format: Book
• Published: Universitas Airlangga, 2008-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<span style="font-family: TribuneBold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Background</strong><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>: Lipid peroxidation (LPO) has implication in pathogenesis of several pathological disorders including periodontitis. </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Malondialdehyde (MDA) is end products of upid peroxidation. Hyperbaric Oxygen Therapy (HBOT) involves the administration of </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>100% oxygen under atmosphere pressure and has been used as an adjuvant therapy, while saliva is a diagnostic tool for many oral </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>and systemic diseases. </em><span style="font-family: TribuneBold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Purpose</strong><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>: The aim of this study was to examine the effect of HBOT on malondialdehyde in saliva to measure </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>lipid peroxidation in periodontitis patients with type 2 Diabetes Mellitus (DM). </em><span style="font-family: TribuneBold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Methods</strong><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>: Eight regulated type 2 DM subjects were </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>compared to ten unregulated periodontitis patients type 2 DM (n = 18). Pre HBOT and after 10 days HBOT with 2.4 ATA dose, </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>unstimulated whole saliva samples from study subjects were collected, centrifuged at 3000 g for 15 minutes and were then stored at </em><span style="font-family: SymbolProportionalBT-Regular; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;">−<span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>80° C until analyzed. The MDA level was determined with 2-thiobarbituric acid by a colorimetric method at 532 nm. </em><span style="font-family: TribuneBold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Results</strong><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>: Data </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>showed that regulated type 2 DM had lower level of MDA (3.08 ± 0.62 ug/mol) compared with unregulated periodontally- type 2 </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>DM subjects (5.88 ± 1.04 ug/mol) (p>0.05). MDA levels were significantly lower after HBOT in regulated DM (2.30 ± 0.46 ug/mol) </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>compared with unregulated periodontally type 2 DM (4.09 ± 0.77ug/mol) (p < 0.05). The regulated DM subjects and post HBOT </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>showed MDA levels lower than the periodontally-unregulated group significantly. </em><span style="font-family: TribuneBold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Conclusion</strong><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>: The saliva of periodontitis patients </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>with unregulated type 2 DM showed more lipid peroxidation than regulated DM type 2. HBOT decreased MDA levels in regulated </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>and unregulated type 2 DM with periodontitis.</em></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span>

Item Description

1978-3728
2442-9740
10.20473/j.djmkg.v41.i4.p151-154