Direct Derivatization in Dried Blood Spots for Oxidized and Reduced Glutathione Quantification in Newborns

• Main Authors: Isabel Ten-Doménech (Author), Álvaro Solaz-García (Author), Inmaculada Lara-Cantón (Author), Alejandro Pinilla-Gonzalez (Author), Anna Parra-Llorca (Author), Máximo Vento (Author), Guillermo Quintás (Author), Julia Kuligowski (Author)
• Format: Book
• Published: MDPI AG, 2022-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The glutathione (GSH)-to-glutathione disulfide (GSSG) ratio is an essential node contributing to intracellular redox status. GSH/GSSG determination in whole blood can be accomplished by liquid chromatography-mass spectrometry (LC-MS) after the derivatization of GSH with <i>N</i>-ethylmal...