Exploiting next-generation sequencing in antibody selections - a simple PCR method to recover binders

• Main Authors: Fortunato Ferrara (Author), Andre A. Teixeira (Author), Leslie Naranjo (Author), M. Frank Erasmus (Author), Sara D'Angelo (Author), Andrew R.M. Bradbury (Author)
• Format: Book
• Published: Taylor & Francis Group, 2020-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Antibody discovery using invitro display technologies such as phage and/or yeast display has become acornerstone in many research and development projects, including the creation of new drugs for clinical use. Traditionally, after the selection phase, random clones are isolated for binding validatio...