The SNAP-tag technology revised: an effective chemo-enzymatic approach by using a universal azide-based substrate

• Main Authors: Rosa Merlo (Author), Diego Caprioglio (Author), Michele Cillo (Author), Anna Valenti (Author), Rosanna Mattossovich (Author), Castrese Morrone (Author), Alberto Massarotti (Author), Franca Rossi (Author), Riccardo Miggiano (Author), Antonio Leonardi (Author), Alberto Minassi (Author), Giuseppe Perugino (Author)
• Format: Book
• Published: Taylor & Francis Group, 2021-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

SNAP-tag® is a powerful technology for the labelling of protein/enzymes by using benzyl-guanine (BG) derivatives as substrates. Although commercially available or ad hoc produced, their synthesis and purification are necessary, increasing time and costs. To address this limitation, here we suggest a...