Transcriptome-wide Cas13 guide RNA design for model organisms and viral RNA pathogens

• Main Authors: Xinyi Guo (Author), Jahan A. Rahman (Author), Hans-Hermann Wessels (Author), Alejandro Méndez-Mancilla (Author), Daniel Haro (Author), Xinru Chen (Author), Neville E. Sanjana (Author)
• Format: Book
• Published: Elsevier, 2021-10-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary: The recent characterization of RNA-targeting CRISPR nucleases has enabled diverse transcriptome engineering and screening applications that depend crucially on prediction and selection of optimized CRISPR guide RNAs (gRNAs). Previously, we developed a computational model to predict RfxCas13...