Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay

• Hauptverfasser: Tshiphiri Senamela (Verfasst von), Marleen Kock (Verfasst von), Piet Becker (Verfasst von), Joachim J.C. Potgieter (Verfasst von)
• Format: Buch
• Veröffentlicht: AOSIS, 2018-01-01T00:00:00Z.
• Schlagworte: article
• Online-Zugang: Connect to this object online.

The purpose of this study was to develop a real time polymerase chain reaction (PCR) assay for the detection of the JAK2 V617F mutation that could be used in diagnostic laboratories. Sanger sequencing and a newly developed locked nucleic-acid, real-time PCR assay were used to detect the JAK2 V617F m...