Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay

• Autori principali: Tshiphiri Senamela (Autore), Marleen Kock (Autore), Piet Becker (Autore), Joachim J.C. Potgieter (Autore)
• Natura: Libro
• Pubblicazione: AOSIS, 2018-01-01T00:00:00Z.
• Soggetti: article
• Accesso online: Connect to this object online.

The purpose of this study was to develop a real time polymerase chain reaction (PCR) assay for the detection of the JAK2 V617F mutation that could be used in diagnostic laboratories. Sanger sequencing and a newly developed locked nucleic-acid, real-time PCR assay were used to detect the JAK2 V617F m...