Effects of Growth Medium and Inoculum Size on Pharmacodynamics Activity of Marbofloxacin against <i>Staphylococcus aureus</i> Isolated from Caprine Clinical Mastitis
<i>Staphylococcus aureus</i> (<i>S. aureus</i>) is an important pathogen that causes clinical mastitis in goats and produces infections difficult to cure. Different antimicrobials as fluoroquinolones have been used against <i>S. aureus</i>. However, the studies de...
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| Main Authors: | , , , , , |
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| Format: | Book |
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MDPI AG,
2021-10-01T00:00:00Z.
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| Summary: | <i>Staphylococcus aureus</i> (<i>S. aureus</i>) is an important pathogen that causes clinical mastitis in goats and produces infections difficult to cure. Different antimicrobials as fluoroquinolones have been used against <i>S. aureus</i>. However, the studies developed to evaluate the bacterial drug interaction only have used the MIC as a single reference point with artificial growth media. The aims of this study were to describe the effect of marbofloxacin on <i>S. aureus</i> isolated from mastitis goats' milk by different approaches as the minimum inhibitory and bactericidal concentrations (MIC and MBC) in cation adjusted Mueller-Hinton broth (CAMHB), serum and milk of goats at two inoculum sizes of 10<sup>5</sup> and 10<sup>8</sup> CFU/mL, the determination and analysis of the time kill curves (TKC) by non-linear mixed effect models in each growth medium and inoculum size, as well as the estimation of their pharmacokinetics/pharmacodynamics (PK/PD) cutoff values. The results obtained indicate that MIC values were higher and increases 2,4-fold in serum and 3,6-fold in milk at high inoculum, as well as the EC<sub>50</sub> values determined by each pharmacodynamics model. Finally, the PK/PD cutoff values defined as fAUC<sub>24</sub>/MIC ratios to achieve clinical efficacy were highly dependent on inoculum and growth medium, with median values of 60-180, especially at high inoculum in milk, suggesting that further studies are necessary to evaluate and optimize the best therapeutic strategies for treating <i>S. aureus</i> in lactating goats. |
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| Item Description: | 10.3390/antibiotics10111290 2079-6382 |