DROSHA Knockout Leads to Enhancement of Viral Titers for Vectors Encoding miRNA-Adapted shRNAs

• Main Authors: Hee Ho Park (Author), Robinson Triboulet (Author), Martin Bentler (Author), Swaroopa Guda (Author), Peng Du (Author), Haiming Xu (Author), Richard I. Gregory (Author), Christian Brendel (Author), David A. Williams (Author)
• Format: Book
• Published: Elsevier, 2018-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

RNAi-based gene therapy using miRNA-adapted short hairpin RNAs (shRNAmiR) is a powerful approach to modulate gene expression. However, we have observed low viral titers with shRNAmiR-containing recombinant vectors and hypothesized that this could be due to cleavage of viral genomic RNA by the endoge...