Integration of Cardiac Actin Mutants Causing Hypertrophic (p.A295S) and Dilated Cardiomyopathy (p.R312H and p.E361G) into Cellular Structures

• Main Authors: Constanze Erdmann (Author), Roua Hassoun (Author), Sebastian Schmitt (Author), Carlos Kikuti (Author), Anne Houdusse (Author), Antonina J. Mazur (Author), Andreas Mügge (Author), Nazha Hamdani (Author), Matthias Geyer (Author), Kornelia Jaquet (Author), Hans Georg Mannherz (Author)
• Format: Book
• Published: MDPI AG, 2021-07-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The human mutant cardiac α-actins p.A295S or p.R312H and p.E361G, correlated with hypertrophic or dilated cardiomyopathy, respectively, were expressed by the <i>baculovirus/Sf21</i> insect cell system and purified to homogeneity. The purified cardiac actins maintained their native state...