Development of 2, 7-Diamino-1, 8-Naphthyridine (DANP) Anchored Hairpin Primers for RT-PCR Detection of Chikungunya Virus Infection.

• Main Authors: Huixin Chen (Author), Mariya Parimelalagan (Author), Fumie Takei (Author), Hapuarachchige Chanditha Hapuarachchi (Author), Evelyn Siew-Chuan Koay (Author), Lee Ching Ng (Author), Phui San Ho (Author), Kazuhiko Nakatani (Author), Justin Jang Hann Chu (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2016-08-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

A molecular diagnostic platform with DANP-anchored hairpin primer was developed and evaluated for the rapid and cost-effective detection of Chikungunya virus (CHIKV) with high sensitivity and specificity. The molecule 2, 7-diamino-1, 8-naphthyridine (DANP) binds to a cytosine-bulge and emits fluores...