High-throughput genotyping of a full voltage-gated sodium channel gene via genomic DNA using target capture sequencing and analytical pipeline MoNaS to discover novel insecticide resistance mutations.

• Main Authors: Kentaro Itokawa (Author), Tsuyoshi Sekizuka (Author), Yoshihide Maekawa (Author), Koji Yatsu (Author), Osamu Komagata (Author), Masaaki Sugiura (Author), Tomonori Sasaki (Author), Takashi Tomita (Author), Makoto Kuroda (Author), Kyoko Sawabe (Author), Shinji Kasai (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2019-11-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

In insects, the voltage-gated sodium channel (VGSC) is the primary target site of pyrethroid insecticides. Various amino acid substitutions in the VGSC protein, which are selected under insecticide pressure, are known to confer insecticide resistance. In the genome, the VGSC gene consists of more th...