Transient, flexible gene editing in zebrafish neutrophils and macrophages for determination of cell-autonomous functions

• Main Authors: Abdulsalam I. Isiaku (Author), Zuobing Zhang (Author), Vahid Pazhakh (Author), Harriet R. Manley (Author), Ella R. Thompson (Author), Lucy C. Fox (Author), Satwica Yerneni (Author), Piers Blombery (Author), Graham J. Lieschke (Author)
• Format: Book
• Published: The Company of Biologists, 2021-07-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Zebrafish are an important model for studying phagocyte function, but rigorous experimental systems to distinguish whether phagocyte-dependent effects are neutrophil or macrophage specific have been lacking. We have developed and validated transgenic lines that enable superior demonstration of cell-...