Monitoring of complex forming of the active pharmaceutical substance and β-cyclodextrin as an additive of the mobile phase using mass spectrometry

• Główni autorzy: Milenković Anđela (Autor), Miljić Dragana (Autor), Mitrović Jelena (Autor), Protić Ana (Autor), Otašević Biljana (Autor)
• Format: Książka
• Wydane: Pharmaceutical Association of Serbia, Belgrade, Serbia, 2016-01-01T00:00:00Z.
• Hasła przedmiotowe: article
• Dostęp online: Connect to this object online.
• Notes: 0004-1963; 2217-8767; 10.5937/arhfarm1604147M
• Keywords: mass spectrometry; inclusion complex; beta-cyclodextrins; sartans; Pharmacy and materia medica; RS1-441
• Source: Arhiv za farmaciju, Vol 66, Iss 4, Pp 147-160 (2016)

In recent years, there is aim to reduce toxic solvents use in chromatographic techniques. Beta-cyclodextrins (β-CD) are commonly used for this purpose, as substance retention modulators. By increasing their concentration in the mobile phase, generally there is a decrease in analyte retention time, and consequently, a decrease in the organic solvent ratio in the mobile phase. The aim of this study was to investigate β-CD and selected sartans inclusion complexes formation. The emphasis was on the influence of the aqueous phase pH value, the drug-β-CD molar ratio and the organic solvent type on complex formation. The mass spectrometry investigation included characterization of the formed complex drug: β-CD at different molar ratios (1:1, 1:2, 1:5 and 1:10), and at different pH values of the aqueous phase (5, 7 and 9) using triple quadrupole mass analyzer. The mobile phase and solvent in which the complexes were formed consisted of acetonitrile and water mixture (50:50, v/v) and methanol and water mixture (50:50, v/v). Measurements of these complexes signal intensity after electrospray ionization in negative mode. Observed m/z values indicated formation of drug-β-CD in 1:1 ratio at all pH values of water phase and independently of organic solvent type and β-CD ratio. It was noted that formed complex signal intensity increased only by increasing β-CD ratio.