Multiple mutations in heterogeneous miltefosine-resistant Leishmania major population as determined by whole genome sequencing.

• Main Authors: Adriano C Coelho (Author), Sébastien Boisvert (Author), Angana Mukherjee (Author), Philippe Leprohon (Author), Jacques Corbeil (Author), Marc Ouellette (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2012-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Miltefosine (MF) is the first oral compound used in the chemotherapy against leishmaniasis. Since the mechanism of action of this drug and the targets of MF in Leishmania are unclear, we generated in a step-by-step manner Leishmania major promastigote mutants highly resistant to MF. Two of the mutan...