Molecular typing of fumC, icd, and mdh genes in Serratia Marcescens

• Main Author: Alaa S Hamzah (Author)
• Format: Book
• Published: Wolters Kluwer Medknow Publications, 2023-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 0975-7406; 10.4103/jpbs.jpbs_93_23
• Keywords: fumc gene; icd gene; mdh gene; tca cycle; typing; Pharmacy and materia medica; RS1-441; Analytical chemistry; QD71-142
• Source: Journal of Pharmacy and Bioallied Sciences, Vol 15, Iss 6, Pp 871-874 (2023)

Aim: Serratia marcescens genes fumC, icd, and mdh were molecularly typed in various groups of 200 clinical samples. Results: According to the findings, 38 (19%) of the isolates are Serratia marcescens. All these bacterial isolates had their DNA extracted. Then, using particular primers, the genes fumC, icd, and mdh are detected and amplified. These genes were sequenced, and the results were aligned with NCBI sequences. Using the Geneious version 9 software, gene sequences were analyzed. Sequencing of these genes revealed variant regions when compared to global isolates in NCBI. Energy levels in bacterial cells may be impacted by TCA cycle enzyme variant sequence genes. Conclusion: The bacterial sequences from Iraq that were listed in NCBI with an accession number were LC735551 is a gene bank. (Gene Bank: LC735550, 1 Iraqi 40 fumC gene. One Iraq 41 icd gene; gene accession number: LC735549.42 mdh gene in Iraq.)