Impact of SARS-CoV-2 Mutations on PCR Assay Sequence Alignment

• Main Authors: Daniel Antonio Negrón (Author), June Kang (Author), Shane Mitchell (Author), Mitchell Y. Holland (Author), Stephen Wist (Author), Jameson Voss (Author), Lauren Brinkac (Author), Katharine Jennings (Author), Stephanie Guertin (Author), Bruce G. Goodwin (Author), Shanmuga Sozhamannan (Author)
• Format: Book
• Published: Frontiers Media S.A., 2022-04-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Real-time reverse transcription polymerase chain reaction (RT-PCR) assays are the most widely used molecular tests for the detection of SARS-CoV-2 and diagnosis of COVID-19 in clinical samples. PCR assays target unique genomic RNA regions to identify SARS-CoV-2 with high sensitivity and specificity....