Rationally designed mariner vectors for functional genomic analysis of Actinobacillus pleuropneumoniae and other Pasteurellaceae species by transposon-directed insertion-site sequencing (TraDIS)

• المؤلفون الرئيسيون: Janine T. Bossé (مؤلف), Yanwen Li (مؤلف), Leon G. Leanse (مؤلف), Liqing Zhou (مؤلف), Roy R. Chaudhuri (مؤلف), Sarah E. Peters (مؤلف), Jinhong Wang (مؤلف), Gareth A. Maglennon (مؤلف), Matthew T. G. Holden (مؤلف), Duncan J. Maskell (مؤلف), Alexander W. Tucker (مؤلف), Brendan W. Wren (مؤلف), Andrew N. Rycroft (مؤلف), Paul R. Langford (مؤلف), on behalf of the BRaDP1T consortium (مؤلف)
• التنسيق: كتاب
• منشور في: BMC, 2021-11-01T00:00:00Z.
• الموضوعات: article
• الوصول للمادة أونلاين: Connect to this object online.

Abstract Comprehensive identification of conditionally essential genes requires efficient tools for generating high-density transposon libraries that, ideally, can be analysed using next-generation sequencing methods such as Transposon Directed Insertion-site Sequencing (TraDIS). The Himar1 (mariner...