Rationally designed mariner vectors for functional genomic analysis of Actinobacillus pleuropneumoniae and other Pasteurellaceae species by transposon-directed insertion-site sequencing (TraDIS)

• Main Authors: Janine T. Bossé (Author), Yanwen Li (Author), Leon G. Leanse (Author), Liqing Zhou (Author), Roy R. Chaudhuri (Author), Sarah E. Peters (Author), Jinhong Wang (Author), Gareth A. Maglennon (Author), Matthew T. G. Holden (Author), Duncan J. Maskell (Author), Alexander W. Tucker (Author), Brendan W. Wren (Author), Andrew N. Rycroft (Author), Paul R. Langford (Author), on behalf of the BRaDP1T consortium (Author)
• Format: Book
• Published: BMC, 2021-11-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Abstract Comprehensive identification of conditionally essential genes requires efficient tools for generating high-density transposon libraries that, ideally, can be analysed using next-generation sequencing methods such as Transposon Directed Insertion-site Sequencing (TraDIS). The Himar1 (mariner...