Generation of Leishmania hybrids by whole genomic DNA transformation.

• Ngā kaituhi matua: Adriano C Coelho (Author), Philippe Leprohon (Author), Marc Ouellette (Author)
• Hōputu: Pukapuka
• I whakaputaina: Public Library of Science (PLoS), 2012-01-01T00:00:00Z.
• Ngā marau: article
• Urunga tuihono: Connect to this object online.

Genetic exchange is a powerful tool to study gene function in microorganisms. Here, we tested the feasibility of generating Leishmania hybrids by electroporating genomic DNA of donor cells into recipient Leishmania parasites. The donor DNA was marked with a drug resistance marker facilitating the se...