<i>Hermetia illucens</i> Fermented with <i>Lactobacillus plantarum</i> KCCM12757P Alleviates Dextran Sodium Sulfate-Induced Colitis in Mice

• Main Authors: Seok Jun Son (Author), Ah-Ram Han (Author), Mi Jeong Sung (Author), Sun Mee Hong (Author), Sang-Hee Lee (Author)
• Format: Book
• Published: MDPI AG, 2023-10-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Inflammatory bowel disease (IBD) can severely affect humans and animals and is difficult to treat. Black soldier fly (<i>Hermetia illucens</i>; Hi) larvae (BSFL) are a sustainable source of protein. However, no studies exist on the antioxidant and anti-inflammatory functions of BSFL or fermented BSFL with respect to IBD. In this study, riboflavin-producing <i>Lactobacillus plantarum</i> KCCM12757P was isolated from a fish farm tank, and in conjunction with hot water-extracted Hi (HeHi) (termed HeHi_Lp), was used to determine optimal fermentation conditions to increase vitamin B₂ concentration. This in vivo study investigated the therapeutic effects and mechanistic role of HeHi_Lp in chronic colitis-induced murine models. Histological changes, inflammatory cytokine levels, and intestinal barrier function were explored. Gut microbial communities and gene expression in the nuclear factor (NF)-κB signaling pathway were also studied. HeHi_Lp remarkably reduced the disease activity index, inflammatory cytokine (inducible nitric oxide synthase, cyclooxygenase 2, tumor necrosis factor α, interleukin (IL-6 and IL-1β) levels, and increased body weight and colon length. HeHi_Lp administration significantly raised zonula occludens 1, occludin and claudin 1 and improved the composition of the gut microbiota and beneficial intestinal bacteria. These results suggest that HeHi_Lp can be used as a dietary supplement in pet food to alleviate colitis.

Item Description

10.3390/antiox12101822
2076-3921