Enumeration of Mycobacterium leprae using real-time PCR.

• Main Authors: Richard W Truman (Author), P Kyle Andrews (Author), Naoko Y Robbins (Author), Linda B Adams (Author), James L Krahenbuhl (Author), Thomas P Gillis (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2008-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Mycobacterium leprae is not cultivable in axenic media, and direct microscopic enumeration of the bacilli is complex, labor intensive, and suffers from limited sensitivity and specificity. We have developed a real-time PCR assay for quantifying M. leprae DNA in biological samples. Primers were ident...