Quantifying LAGA mutated mouse IgG2a monoclonal antibody with a rapid pepsin digestion enabled immunoaffinity LC/MS/MS assay

• Egile Nagusiak: Linlin Dong (Egilea), Susan Chen (Egilea), Konstantin Piatkov (Egilea), Dong Wei (Egilea), Mark G. Qian (Egilea)
• Formatua: Liburua
• Argitaratua: Taylor & Francis Group, 2024-12-01T00:00:00Z.
• Gaiak: article
• Sarrera elektronikoa: Connect to this object online.

A sensitive and specific bioanalytical method was required to measure the exposure of a LAGA-mutated surrogate mouse IgG2a monoclonal antibody in mouse plasma, but the lack of highly specific reagents for the LAGA mutant hindered the development of a ligand-binding assay. Equally problematic is that...