Comparative analysis of cell-free DNA content in culture medium and mitochondrial DNA copy number in porcine parthenogenetically activated embryos

• Main Authors: Mitsuru KOBAYASHI (Author), Jun ITO (Author), Koumei SHIRASUNA (Author), Takehito KUWAYAMA (Author), Hisataka IWATA (Author)
• Format: Book
• Published: The Society for Reproduction and Development, 2020-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

We examined the effect of ploidy on mitochondrial DNA (mtDNA) copy number in embryos and the amount of cell-free mitochondrial and nucleic DNA content (cf-mtDNA and cf-nDNA) in spent culture medium (SCM). Oocytes collected from the ovaries were matured, activated, incubated in medium containing cycl...