Effect of the Compaction and the Size of DNA on the Nuclear Transfer Efficiency after Microinjection in Synchronized Cells

• Autors principals: Hidetaka Akita (Autor), Dai Kurihara (Autor), Marco Schmeer (Autor), Martin Schleef (Autor), Hideyoshi Harashima (Autor)
• Format: Llibre
• Publicat: MDPI AG, 2015-06-01T00:00:00Z.
• Matèries: article
• Accés en línia: Connect to this object online.

The nuclear transfer process is one of the critical rate-limiting processes in transgene expression. In the present study, we report on the effect of compaction and the size of the DNA molecule on nuclear transfer efficiency by microinjection. A DNA/protamine complex- or variously-sized naked DNA mo...