Pulp tissue vacuolization and necrosis after direct pulp capping with calcium hydroxide and transforming growth factor-β1

• Main Author: Sri Kunarti (Author)
• Format: Book
• Published: Universitas Airlangga, 2008-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 1978-3728; 2442-9740; 10.20473/j.djmkg.v41.i1.p35-38
• Keywords: direct pulp capping; Ca(OH)2; TGF-β1; vacuolization; necrosis; Dentistry; RK1-715
• Source: Dental Journal, Vol 41, Iss 1, Pp 35-38 (2008)

<span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Mechanical pulp exposure by a rotary cutting instrument or a hand-cutting instrument often happens in deep caries. Application </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>of protective dressing can protect the pulp from additional injury by facilitating healing and repair. Pulp capping has been suggested </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>as one treatment of choice after pulp exposure to maintain pulp vitality. TGF-</em><span style="font-family: SymbolProportionalBT-Regular; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>β1 is growth factor that has important rule in wound </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>healing. The application of Ca(OH)</em><span style="font-family: Tribune-Italic; font-size: 7pt; color: #231f20; font-style: normal; font-variant: normal;"><em>2 </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>and exogenous TGF-</em><span style="font-family: SymbolProportionalBT-Regular; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>β1 as direct pulp capping tr4eatment must be experimented in-vivo to see </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>the vacuolization and necrosis in 7, 14, and 21 days after application. This research was done in vivo experiment from orthodontic </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>patients indicated for premolar extraction, between ages 10-15 years. A class V cavity preparation was created in the buccal aspect </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>1 mm above gingival margin until pulp exposure. Cavity was irrigated slowly with saline solution and dried with a sterile small cotton </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>pellet. Group 1 calcium hydroxide was applied as manufacture procedure. Group 2, the sterile absorbable collagen membrane used, </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>as inert carrier of TGF-</em><span style="font-family: SymbolProportionalBT-Regular; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>β1 was soaked with 5 </em><span style="font-family: SymbolProportionalBT-Regular; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;">m<span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>l. All groups were covered by a Teflon pledge to separate pulp capping agent from glass </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>ionomer cement restoration. Teeth extracted in 7, 14 and 21 days after treatment. All samples were hystopathologically examined. There </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>were significant difference of TGF-</em><span style="font-family: SymbolProportionalBT-Regular; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>β</em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>1 (p < 0.05) in the vacuolization day 14</em><span style="font-family: Tribune-Italic; font-size: 7pt; color: #231f20; font-style: normal; font-variant: normal;"><em>th </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>and 21</em><span style="font-family: Tribune-Italic; font-size: 7pt; color: #231f20; font-style: normal; font-variant: normal;"><em>th </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>compared with 7</em><span style="font-family: Tribune-Italic; font-size: 7pt; color: #231f20; font-style: normal; font-variant: normal;"><em>th</em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>. there were not significant </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>difference in necrosis for all variables. Vacuolization and necrosis decreased in the application of TGF-</em><span style="font-family: SymbolProportionalBT-Regular; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>β</em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>1.</em></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span>