Development and validation of portable, field-deployable Ebola virus point-of-encounter diagnostic assay for wildlife surveillance

• Main Authors: Dania M. Figueroa (Author), Eeva Kuisma (Author), M. Jeremiah Matson (Author), Alain U. Ondzie (Author), Trent Bushmaker (Author), Stephanie N. Seifert (Author), Francine Ntoumi (Author), Beatriz Escudero-Pérez (Author), César Muñoz-Fontela (Author), Chris Walzer (Author), Sarah H. Olson (Author), Cynthia Goma-Nkoua (Author), Jean-Vivien Mombouli (Author), Robert J. Fischer (Author), Vincent J. Munster (Author)
• Format: Book
• Published: BMC, 2021-05-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Abstract Early detection of Ebola virus spillover into wildlife is crucial for rapid response. We developed and validated a portable, cold-chain independent Ebola virus RT-qPCR assay. Methods The field syringe-based RNA extraction method was compared with a conventional laboratory-based spin-column...