Skeletal muscle triad junction ultrastructure by Focused-Ion-Beam milling of muscle and Cryo-Electron Tomography

• Autores principales: Terence Wagenknecht (Autor), Chyongere Hsieh (Autor), Michael Marko (Autor)
• Formato: Libro
• Publicado: PAGEPress Publications, 2015-01-01T00:00:00Z.
• Materias: article
• Acceso en línea: Connect to this object online.

Cryo-electron tomography (cryo-ET) has emerged as perhaps the only practical technique for revealing nanometer-level three-dimensional structural details of subcellular macromolecular complexes in their native context, inside the cell. As currently practiced, the specimen should be 0.1- 0.2 microns...