Canine skin and conjunctival swab samples for the detection and quantification of Leishmania infantum DNA in an endemic urban area in Brazil.

• Main Authors: Sidney de Almeida Ferreira (Author), Rodrigo Souza Leite (Author), Leonardo Trindade Ituassu (Author), Gregório Guilherme Almeida (Author), Daniel Menezes Souza (Author), Ricardo Toshio Fujiwara (Author), Antero Silva Ribeiro de Andrade (Author), Maria Norma Melo (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2012-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

<h4>Background</h4>We evaluated kDNA PCR/hybridization and quantitative real-time PCR (qPCR) targeting the gene of DNA polymerase of Leishmania infantum for CVL diagnosis and assessment of parasite load in clinical samples obtained invasively and non-invasively.<h4>Methodology/prin...