New Method of Isothermal, Hairpin Assisted, Primer Independent Amplification of DNA

• Main Authors: Denis Sergeevich Naberezhnov (Author), Alexander Andreevich Alferov (Author), Yuriy Borisovich Kuzmin (Author), Nikolay Evgenievich Kushlinskii (Author)
• Format: Book
• Published: MDPI AG, 2023-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The isothermal amplification of nucleic acids refers to processes that quickly increase the amount of DNA at a constant temperature. These methods are mainly developed as alternatives to PCR for cases in which the application of a thermal cycler is not possible or the assay method must be as rapid a...